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The Inflammasome 1st Edition by Ali A Abdul Sater ISBN 1071621432 9781071621431

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The Inflammasome 1st Edition Ali A. Abdul-Sater Digital Instant Download

Author(s): Ali A. Abdul-Sater
ISBN(s): 9781071621431, 1071621432
Edition: 1st
File Details: PDF, 4.24 MB
Year: 2022
Language: english
SKU: EB-47937142 Category: Tag:

The Inflammasome 1st Edition by Ali A. Abdul-Sater – Ebook PDF Instant Download/Delivery: 1071621432, 9781071621431

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Product details:

ISBN 10: 1071621432

ISBN 13:  9781071621431

Author: Ali A. Abdul-Sater

This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible prools.

Authoritative and cutting-edge, The Inflammasome: Methods and Prools aims to provide prools that will serve as tools for current and aspiring researchers interested in studying inflammasomes.

Table of contents:

  1. Assessment of ASC Oligomerization by Flow Cytometry

  2. In-vitro Assays to Study Inflammasome Activation in Primary Macrophages

  3. Measuring Non-Canonical Inflammasome Activation in Neutrophils

  4. Gasdermin D Cleavage Assay Following Inflammasome Activation

  5. Activation of the Non-Canonical Inflammasome in Mouse and Human Cells

  6. Measurement of Inflammasome-Induced Mitochondrial Dysfunction by Flow Cytometry

  7. Detection of ASC Oligomerization by Western Blotting

  8. Reconstitution System of NLRP3 Inflammasome in HEK293T Cells

  9. Intracellular Potassium Ions Measurements by ICP-OES

  10. NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots

  11. Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood

  12. Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation

  13. Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy

  14. In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 Inflammasome Activation

  15. Inflammasome Activation in Gingival Epithelial Cells

  16. Imaging of Inflammasome Speck Formation in Living Cells

 

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Tags: Ali A Abdul Sater, Inflammasome