Tiling Arrays Methods and Protocols 1st Edition by Tin Lap Lee, Alfred Chun Shui Luk ISBN 1493959921 9781493959921

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Product details:

ISBN 10: 1493959921

ISBN 13: 978-1493959921

Author:  Tin-Lap Lee, Alfred Chun Shui Luk 

Tiling Arrays: Methods and Protocols 1st Edition: 

 In Tiling Arrays: Methods and Protocols,  expert researchers in the field detail many of the methods which are now commonly used to study tiling microarrays in genomic discovery . These include methods and applications for transcriptional regulation, expression, genetic and epigenetic regulation, as well as techniques and skills on tiling microarray data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tiling Arrays: Methods and Protocols  seeks to provide scientists with a comprehensive and down to earth approach to maximize results.

 

Tiling Arrays: Methods and Protocols 1st Edition Table of contents:

• Overview

  • A brief introduction to tiling microarrays: principles, concepts, and applications

• Transcriptional regulation and expression

  • Design of tiling arrays and their application to bacterial transcriptome analysis
  • Transcript profiling in Arabidopsis with genome tiling microarrays
  • Genome-wide analysis of transcription factor-binding sites in skeletal muscle cells using ChIP-Seq
  • Analysis of allele-specific gene expression using a target-oriented tiling microarray assay

• Genetics and epigenetics regulation

  • Detection of epigenetic alterations using tiling arrays
  • Investigating gene promoter methylation in a mouse model of status epilepticus

• Data analysis

  • Integrative analysis of ChIP-Chip and ChIP-Seq dataset
  • HAT: a novel statistical approach to discover functional regions in the genome
  • Inference of alternative splicing from tiling array data
  • Analysis of in vivo occupancy of Aebp1, a transcription factor, using high-resolution tiling array
  • Application of the simple and efficient Mpeak modeling in binding peak identification in ChIP-Chip studies
  • Evaluation of MeDIP-Chip in the context of whole-genome bisulfite sequencing (WGBS-Seq) in Arabidopsis
  • Mapping genomic features of tiling microarray data by TileMapper

 

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Tin Lap Lee,Alfred Chun Shui Luk,Tiling,Arrays,Methods,Protocols