Sale!

Transgenic Maize Methods and Protocols 1st Edition by Dong Liu, M Paul Scott ISBN 1934115495 9781934115497

Original price was: $50.00.Current price is: $35.00.

Transgenic Maize Methods and Protocols 1st Edition Dong Liu (Auth.) Digital Instant Download

Author(s): Dong Liu (auth.), M. Paul Scott (eds.)
ISBN(s): 9781934115497, 1934115495
Edition: 1
File Details: PDF, 2.16 MB
Year: 2009
Language: english
SKU: EB-2111604 Category: Tags: ,

Transgenic Maize Methods and Protocols 1st Edition by Dong Liu, M. Paul Scott – Ebook PDF Instant Download/Delivery: 1934115495, 9781934115497

Full download Transgenic Maize Methods and Protocols 1st Edition after payment

 

Product details:

ISBN 10: 1934115495

ISBN 13: 9781934115497 

Author: Dong Liu, M. Paul Scott 

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology#x99; series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Table of contents:

  1. Design of Gene Constructs for Transgenic Maize

  2. Transient Expression of GFP in Immature Seed Tissues

  3. Biolistic Gun-Mediated Maize Genetic Transformation

  4. Agrobacterium-Mediated Maize Transformation: Immature Embryos versus Callus

  5. Whiskers-Mediated Maize Transformation

  6. Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines

  7. Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression

  8. Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites

  9. Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize

  10. Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds

  11. Determination of Transgene Copy Number by Real-Time Quantitative PCR

  12. Herbicide Resistance Screening Assay

  13. Characterizing Transgene Inheritance

  14. Backcross Breeding

People also search for:

transgenic maize methods and protocols
    
transgenic maize in mexico
    
transgenic corn
    
maize transformation
    
transgenic corn mexico
    
a transgenic animal

Tags: Dong Liu, M Paul Scott, Transgenic, Maize, Methods, Protocols