Ubiquitin Family Modifiers and the Proteasome Reviews and Protocols 1st Edition by Jürgen Dohmen, Martin Scheffner ISBN 1617794740 9781617794742

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Product details:

ISBN 10: 1617794740 

ISBN 13: 9781617794742

Author:  R. Jürgen Dohmen, Martin Scheffner 

Ubiquitin Family Modifiers and the Proteasome: Reviews and Protocols 1st Edition: 

The ubiquitin-proteasome system (UPS) and ubiquitin-related modifiers are not only involved in cellular protein quality control but also in the regulation of many fundamental cellular processes/pathways as well as in their disease-relevant aberrations. Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols presents both novel developments in UPS research and important methods related to the main recent advances in the field of ubiquitin family modifiers. Divided into five convenient sections, this volume focuses on the enzymology and substrate identification of ubiquitin family modifiers, the recognition and chain formation of these modifiers, the analysis of proteasome biogenesis and function, protein quality control, and finally the use of small molecules and strategies to study or manipulate the function of the UPS and of ubiquitin family modifiers, respectively. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols will be of great use to investigators and students engaged in both basic and applied research in life sciences.

 

Ubiquitin Family Modifiers and the Proteasome: Reviews and Protocols 1st Edition Table of contents:

• • Three decades of studies to understand the functions of the ubiquitin family

• Enzymology and substrates of ubiquitin family modifiers

  • Structure and evolution of ubiquitin and ubiquitin-related domains
  • Deciphering tissue-specific ubiquitylation by mass spectrometry
  • Analysis of cellular SUMO and SUMO–ubiquitin hybrid conjugates
  • Recombinant reconstitution of sumoylation reactions in vitro
  • Reconstitution of SUMO-dependent ubiquitylation in vitro
  • Detection and analysis of FAT10 modification
  • Isolation of NEDDylated proteins in human cells
  • The ISG15 conjugation system
  • Pupylation: proteasomal targeting by a protein modifier in bacteria

• Recognition and chain formation of UbF modifiers

  • Role of UbL family modifiers and their binding proteins in cell signaling
  • Isolation of ubiquitylated proteins using tandem ubiquitin-binding entities
  • Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation
  • Measuring rates of ubiquitin chain formation as a functional readout of ligase activity
  • Synthesis and analysis of K11-linked ubiquitin chains
  • Synthesis and analysis of linear ubiquitin chains
  • Detection and quantitation of SUMO chains by mass spectrometry
  • ……………….

 

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Jürgen Dohmen,Martin Scheffner,Ubiquitin,Family,Modifiers,Proteasome,Reviews,Protocols